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Custom Monoclonal Antibody Generation Services

 

Monoclonal antibodies have proven to be instrumental in the advancement of research, diagnostic, industrial vaccine, and therapeutic applications. For therapeutic and diagnostic antibodies, it is essential to be high specificity, high binding affinity, long half-lives and low toxicity whereas antibodies are also an invaluable research resources for antigen detection and purification such as immunoblotting, immunoprecipitation, immunohistochemistry and immunoaffinity chromatography. Generation of monoclonal antibodies require good antigen design, high purity of antigen, strong antibody response from mice, high fusion efficiency and good screening strategy.

 

ImmunoDiagnostics team has over 10 years experience in Monclonal Antibody Generation together with advance technologies to increase the chances for getting the monoclonal antibodies that suit your needs.

 

Mouse monoclonal antibody production is divided into four phases, antigen design and production, mice immunization, hybridoma cell line generation and antibody production. ImmunoDiagnostics offer different valuable packages for monoclonal antibody generation. You can individual join each phase or we can customize according to your needs.

 

 

Why choosing ImmunoDiagnostics

 

Design of Antigen

Our team has substantial experience for antigen and peptide design, which greatly increase the immunogenicity of injected animals.

Antigen Production

We can help to synthesize peptide(s) against your target proteins. Antigen production can be chosen from mammalian cells, insect cells or bacterial cells. Our unique protein expression and purification systems allow us to purify protein in high purity and yield.

Mice Immunization

Our novel immunization procedure allows extra high antibody titer generated by mouse that increases the chance to get the positive clones against your target.

Cell Fusion and Hybridoma cell line generation

Advanced fusion technology is used instead of traditional PEG fusion method to increase the fusion efficiency up to 20 times. Our unique hybridoma cell line screening protocol reduces the chance of missing potential positive clones during the screening process and also speed up the screening process.

Antibody Production

Wide range of monoclonal antibody production service is provided. Antibodies can be generated by mouse ascities fluid or culturing hybridoma cells in serum free medium. Our special protocol and media formulation helps hybridoma cells speedy adapt to the serum free medium that suitable for large scale antibodies production.

 

 

 

Services Details

 

Phase

Package

Deliverable

 

Duration

Phase 1: Antigen Production

  • Synthesize and clone your target genes (with codon optimization) into desire vector for protein expression.
  • Expression and purification of your protein from the selected host.

OR

  • Design and synthesize the peptide against your target protein.

Plasmid synthesis report.

4ug of plasmid.

2mg of purified protein.

 

Peptide synthesized report. 2mg of synthesized peptide

8 weeks

 

 

 

 

 

4 weeks

Phase 2:

Mice Immunization

  • 3 mice are immunized by our novel in-house procedure. (Antibody titer is guaranteed to be >100,000 before cell fusion).

* When Customer provides protein to us; at least 2mg of protein for mice immunization and 1mg screening antigen.

Mice serum test report.

 

8 weeks

Phase 3:

Hybridoma cell line generation

  • Mouse spleenocyte with highest sera titer is fused with myeloma cells.
  • Supernatant from hybridoma cell clones is screened by ELISA.
  •  2 rounds of subcloning are done to confer the colonarity and stability.

** Customer can choose 2 hybridoma clones after according to cell culture test report.

*** Small amount of supernatant can be provided for test upon request.

 

Cell culture test report.

10ml cell culture supernatant of 2 selected clones.

2 hybridoma clones.

6 month of cell banking services.

 

6 weeks

Phase 4:

Antibody production

  • Can choose production of monoclonal antibodies from Serum Free Medium or Mouse Ascites fluid.

 

1mg Protein G purified monoclonal antibodies from 2 selected clones.

8 weeks

 

 

 

Extra Services

 

Services

Details

Deliverable

Duration

Custom screening criteria

  • Extra screening method can be provided for mice sera or cell supernatant from selected clones such as Western Blot, Flow Cytometry, Binding affinity (KD).

Test Report

1 week

Immunizing more mice

  • Immunizing more than 3 mice can increase the chances to get the strong positive mice.

Mice serum test report.

Nil

Additional clones

  • Can choose more hybridoma clones other than default 2 clones.

10ml cell culture supernatant of selected clones.

Selected hybridoma clone.

1mg of purified antibody from selected clones.

Nil

Antibody production

  • Production of extra antibodies from selected clones, default is 1mg/clone.

Purified antibodies

4-8 weeks

Serum Free adaption

  • Adaption of selected hybridoma cell line to serum free medium.
  •  

Serum free media adapted clones.

8-10 weeks

Antibody conjugation

  • Purified antibodies can do conjugation according to your needs.

Conjugated antibodies

2-3 weeks

Cell Banking

  • Extra storage service for your clones, default is 6 months for the selected clones.

N/A

Nil

Hybridoma sequencing

  • Sequencing service for your hybridoma clones variable region by NGS.

Sequencing report for variable region.

2 weeks

 

 

 

Packages and Pricing

 

Package

Details

*Price (HKD)

A

Full Package: Phase 1-4

50,000

B

Customer provide antigen: Phase 2-4

40,000

 

Phase

Details

*Price (HKD)

1

Antigen Production

15,000

2+3

Mice Immunization and hybridoma cell line generation

35,000

4

Antibody production

10,000

 

Extra Service

*Price (HKD)

Custom screening criteria

Contact us

Immunizing more mice

500/mice

Additional clones

2,000/clone

Antibody production

1,000/mg

Serum Free adaption

2,000/clone

Antibody conjugation

5,000/mg

Cell Banking

300/month/clone

Hybridoma sequencing

10,000/clone

 

* Price is for reference only, for details, please contact our customer service representative.

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