Home » Research Products » Recombinant Proteins » Human PM20D1

Human PM20D1

Product Code: 41700

 

Origin:

Recombinant

Cat. No.:

41700

Tag:

N-terminal 6xHis

Size:

0.1 mg

Source:

E.coli

Purity:

>95%

Other Names:

PM20D1

Species:

Human

 

Description

Expressed in E.coli with total 521 AA. Mw: 58.1 kDa (calculated). 
N-terminal 6xHis-tag, EK recognition site and TEV cleavage site, 44 extra AA (highlighted).

Recombinant antigen for research use or manufacturing only.

 

Introduction to the Molecule

PM20D1 is a bidirectional N-fatty-acyl amino acid synthase/hydrolase that regulates the production of N-fatty-acyl amino acids. These metabolites are endogenous chemical uncouplers of mitochondrial respiration. In an UCP1-independent manner, maybe through interaction with mitochondrial transporters, they promote proton leakage into the mitochondrial matrix. PM20D1 may indirectly regulate the bodily dissipation of chemical energy as heat through thermogenic respiration.  

 

Amino Acid Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSENLY FQGAMGPRSG EHQRASRIPS
QFSKEERVAM KEALKGAIQI PTVTFSSEKS NTTALAEFGK YIHKVFPTVV STSFIQHEVV
EEYSHLFTIQ GSDPSLQPYL LMAHFDVVPA PEEGWEVPPF SGLERDGIIY GRGTLDDKNS
VMALLQALEL LLIRKYIPRR SFFISLGHDE ESSGTGAQRI SALLQSRGVQ LAFIVDEGGF
ILDDFIPNFK KPIALIAVSE KGSMNLMLQV NMTSGHSSAP PKETSIGILA AAVSRLEQTP
MPIIFGSGTV VTVLQQLANE FPFPVNIILS NPWLFEPLIS RFMERNPLTN AIIRTTTALT
IFKAGVKFNV IPPVAQATVN FRIHPGQTVQ EVLELTKNIV ADNRVQFHVL SAFDPLPVSP
SDDKALGYQL LRQTVQSVFP EVNITAPVTS IGNTDSRFFT NLTTGIYRFY PIYIQPEDFK
RIHGVNEKIS VQAYETQVKF IFELIQNADT DQEPVSHLHK L

 

Applications

ELISA and Western Blotting.


Formulation

Lyophilized at 1 mg/mL in NaCl 500mM, KCl 2.7mM, Na2HPO4 10mM, KH2PO4 1.8mM, pH 8.0.

 

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.

 

Storage

Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.  Avoid repeated freezing /thawing cycles.

 

Quality Control Test

BCA to determine the quantity of the protein.

SDS PAGE to determine the purity of the protein.

 

SDS-PAGE Gel

 

 

 

 

Contact Us

 

Address:

 

5/F, Biotech Centre 2, No. 11 Science Park West Avenue, 

Hong Kong Science Park, Shatin, N.T., Hong Kong

Email: 

info@immunodiagnostics.com.hk      sales@immunodiagnostics.com.hk

Phone:

(+852) 3502 2780    

Fax:  

(+852)  3502 2781

 

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