Home » Research Products » Recombinant Proteins » Mouse PM20D1

Mouse PM20D1

Product Code: 42701

 

Origin:

Recombinant

Cat. No.:

42701

Tag:

N-terminal 6xHis

Size:

0.1 mg

Source:

E.coli

Purity:

>95%

Other Names:

PM20D1

Species:

Human

 

Description

Expressed in E.coli with total 521 AA. Mw: 58.1 kDa (calculated). 
N-terminal 6xHis-tag, EK recognition site and TEV cleavage site, 44 extra AA (highlighted).

Recombinant antigen for research use or manufacturing only.

 

Introduction to the Molecule

PM20D1 is a bidirectional N-fatty-acyl amino acid synthase/hydrolase that regulates the production of N-fatty-acyl amino acids. These metabolites are endogenous chemical uncouplers of mitochondrial respiration. 
In an UCP1-independent manner, maybe through interaction with mitochondrial transporters, they promote proton leakage into the mitochondrial matrix. 
PM20D1 may indirectly regulate the bodily dissipation of chemical energy as heat through thermogenic respiration.  

 

Amino Acid Sequence

METAELLASL PAWAAVLLLF FATVSGSTGP RSRENRGASR IPSQFSEEER VAIKEALKGA
IQIPTVSFSH EESNTTALAE FGEYIRKAFP TVFHSSLVQH EVVAKYSHLF TIQGSDPSLQ
PYMETLMETA HIDVVPAPEE GWEVPPFSGL ERNGFIYGRG ALDNKNSVME TAILHALELL
LIRNYSPKRS FFIALGHDEE VSGEKGAQKI SALLQARGVQ LAFLVDEGSF ILEGFIPNLE
KPVAMETISV TEKGALDLME TLQVNMETTP GHSSAPPKET SIGILSAAVS RLEQTPMETP
NMETFGGGPL KKTMETKLLA NEFSFPINIV LRNLWLFHPI VSRIMETERN PITNALVRTT
TALTMETFNA GIKVNVIPPL AQATINCRIH PSQTVHEVLE LVKNTVADDR VQLHVLRSFE
PLPISPSDDQ AMETGYQLLQ ETIRSVFPEV DIVVPGICIA NTDTRHYANI TNGMETYRFN
PLPLNPQDFS GVHGINEKVS VQNYQNQVKF IFEFIQNADT YKEPVPHLHE LENLYFQGHH
HHHH

 

Applications

ELISA and Western Blotting.


Formulation

Lyophilized at 1 mg/mL in NaCl 500mM, KCl 2.7mM, Na2HPO4 10mM, KH2PO4 1.8mM, pH 8.0.

 

Endotoxin Level

<0.2 EU per 1 μg of the protein by the LAL method.

 

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.

 

Storage

Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.  Avoid repeated freezing /thawing cycles.

 

Quality Control Test

BCA to determine the quantity of the protein.

SDS PAGE to determine the purity of the protein.

 

SDS-PAGE Gel

 

 

 

 

Contact Us

 

Address:

 

5/F, Biotech Centre 2, No. 11 Science Park West Avenue, 

Hong Kong Science Park, Shatin, N.T., Hong Kong

Email: 

info@immunodiagnostics.com.hk      sales@immunodiagnostics.com.hk

Phone:

(+852) 3502 2780    

Fax:  

(+852)  3502 2781

 

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