Home » Research Products » Recombinant Proteins » Human IL-33

Human IL-33

Product Code: 42750

 

Origin:

Recombinant

Cat. No.:

42750

Tag:

N-terminal 6xHis

Size:

0.1 mg

Source:

E.coli

Purity:

>90%

Other Names:

IL-33

Species:

Human

 

 

Description

Expressed in E.coli with total 167 AA. Mw: 18.81 kDa (calculated). 
No tag, but with 2 extra AA at N-terminal (highlighted).

Recombinant antigen for research use or manufacturing only.

 

Introduction to the Molecule

IL-33 was identified based on sequence and structural homology with IL-1 family cytokines. The N-terminal portion of full-length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses. A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP. Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion. In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues.  

 

Amino Acid Sequence

GASITGISPI TEYLASLSTY NDQSITFALE DESYEIYVED LKKDEKKDKV LLSYYESQHP
SNESGDGVDG KMETLMETVT LSPTKDFWLH ANNKEHSVEL HKCEKPLPDQ AFFVLHNMET
HSNCVSFECK TDPGVFIGVK DNHLALIKVD SSENLCTENI LFKLSET

 

Endotoxin Level

<0.2 EU per 1 μg of the protein by the LAL method.

 

Applications

ELISA and Western Blotting.


Formulation

Lyophilized at 1 mg/mL in PBS.

 

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.

 

Storage

Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.  Avoid repeated freezing/thawing cycles.

 

Quality Control Test

BCA to determine the quantity of the protein.

SDS PAGE to determine the purity of the protein.

 

SDS-PAGE Gel

 

 

 

 

Contact Us

 

Address:

 

5/F, Biotech Centre 2, No. 11 Science Park West Avenue, 

Hong Kong Science Park, Shatin, N.T., Hong Kong

Email: 

info@immunodiagnostics.com.hk      sales@immunodiagnostics.com.hk

Phone:

(+852) 3502 2780    

Fax:  

(+852)  3502 2781

 

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