Home » Research Products » Recombinant Proteins » Human Autotaxin

Human Autotaxin

Product Code: 41771

 

Origin:

Recombinant

Cat. No.:

41771

Tag:

C-terminal 6xHis

Size:

0.1 mg

Source:

HEK293

Purity:

>90%

Other Names:

ATX, ENPP2

Species:

Human

 

 

Description

Expressed in HEK293 cells with total 908 AA. Mw: 104.37 kDa (calculated). 

C-terminal 6xHis-tag, 9 extra AA (highlighted).

Recombinant antigen for research use or manufacturing only.

 

Introduction to the Molecule

Autotaxin (ATX, ENPP2) is a secreted glycoprotein with phosphodiesterase (PDE) activity. It is one of the member in the nucleotide pyrophosphatase/phosphodiesterase family (NPPs) family.

ATX has lysophospholipase D activity that converts lysophosphatidylcholine into LPA, and it was originally identified as a tumor cell-motility-stimulating factor. LPA, which specifically binds to G protein-coupled receptors (GPCR), plays a wide range of biological activities, including cell hyperproliferation, which may contribute to oncogenesis and metastasis.

ATX has been to be overexpressed in many tumor cells and thus plays an important role in tumor development and metastasis. In addition, ATX also plays an important role in nervous and immune systems.

 

Amino Acid Sequence

METARRSSFQ SCQIISLFTF AVGVNICLGF TAHRIKRAEG WEEGPPTVLS DSPWTNISGS
CKGRCFELQE AGPPDCRCDN LCKSYTSCCH DFDELCLKTA RGWECTKDRC GEVRNEENAC
HCSEDCLARG DCCTNYQVVC KGESHWVDDD CEEIKAAECP AGFVRPPLII FSVDGFRASY
METKKGSKVM ETPNIEKLRS CGTHSPYMET RPVYPTKTFP NLYTLATGLY PESHGIVGNS
METYDPVFDA TFHLRGREKF NHRWWGGQPL WITATKQGVK AGTFFWSVVI PHERRILTIL
QWLTLPDHER PSVYAFYSEQ PDFSGHKYGP FGPEMETTNP LREIDKIVGQ LMETDGLKQL
KLHRCVNVIF VGDHGMETED VTCDRTEFLS NYLTNVDDIT LVPGTLGRIR SKFSNNAKYD
PKAIIANLTC KKPDQHFKPY LKQHLPKRLH YANNRRIEDI HLLVERRWHV ARKPLDVYKK
PSGKCFFQGD HGFDNKVNSM ETQTVFVGYG STFKYKTKVP PFENIELYNV METCDLLGLK
PAPNNGTHGS LNHLLRTNTF RPTMETPEEV TRPNYPGIME TYLQSDFDLG CTCDDKVEPK
NKLDELNKRL HTKGSTEERH LLYGRPAVLY RTRYDILYHT DFESGYSEIF LMETPLWTSY
TVSKQAEVSS VPDHLTSCVR PDVRVSPSFS QNCLAYKNDK QMETSYGFLF PPYLSSSPEA
KYDAFLVTNM ETVPMETYPA FKRVWNYFQR VLVKKYASER NGVNVISGPI FDYDYDGLHD
TEDKIKQYVE GSSIPVPTHY YSIITSCLDF TQPADKCDGP LSVSSFILPH RPDNEESCNS
SEDESKWVEE LMETKMETHT ARVRDIEHLT SLDFFRKTSR SYPEILTLKT YLHTYESEIS
YYHHHHHH

 

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

 

Applications

ELISA and Western Blotting.


Formulation

Lyophilized at 1 mg/mL in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).

 

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.

 

Storage

Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.  Avoid repeated freezing/thawing cycles.

 

Quality Control Test

BCA to determine the quantity of the protein.

SDS PAGE to determine the purity of the protein.

 

SDS-PAGE Gel

 

 

 

 

Contact Us

 

Address:

 

5/F, Biotech Centre 2, No. 11 Science Park West Avenue, 

Hong Kong Science Park, Shatin, N.T., Hong Kong

Email: 

info@immunodiagnostics.com.hk      sales@immunodiagnostics.com.hk

Phone:

(+852) 3502 2780    

Fax:  

(+852)  3502 2781

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