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Human Annexin

Product Code: 41720

 

Origin:

Recombinant

Cat. No.:

41720

Tag:

N-terminal 6xHis

Size:

0.1 mg

Source:

E.coli

Purity:

>90%

Other Names:

ANXA8

Species:

Human

 

 

Description

Expressed in E.coli cells with total 392 AA. Mw: 44.35 kDa (calculated). 

N-terminal 6xHis-tag, EK and TEV cleavage site, 46 extra AA (highlighted).

Recombinant antigen for research use or manufacturing only.

 

Introduction to the Molecule

Annexin A8 (ANXA8) belongs to the annexin family of evolutionally conversed Ca2+ and phospholipid binding proteins. ANXA8 may function as an anticoagulant which inhibits the function of thromboplastin-specific complex in an indirect way. Overexpression of this gene has been shown to be associated with acute myelocytic leukemia.

Human ANXA8 shares 92.661% sequence identity with rat ANXA8 and 92.094% with mouse ANXA8.

 

Amino Acid Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSLEEN LYFQGAAWWK SWIEQEGVTV
KSSSHFNPDP DAETLYKAME TKGIGTNEQA IIDVLTKRSN TQRQQIAKSF KAQFGKDLTE
TLKSELSGKF ERLIVALMET YPPYRYEAKE LHDAMETKGL GTKEGVIIEI LASRTKNQLR
EIMETKAYEE DYGSSLEEDI QADTSGYLER ILVCLLQGSR DDVSSFVDPG LALQDAQDLY
AAGEKIRGTD EMETKFITIL CTRSATHLLR VFEEYEKIAN KSIEDSIKSE THGSLEEAME
TLTVVKCTQN LHSYFAERLY YAMETKGAGT RDGTLIRNIV SRSEIDLNLI KCHFKKMETY
GKTLSSMETI METEDTSGDY KNALLSLVGS DP

 

Endotoxin Level

<0.2 EU per 1 μg of the protein by the LAL method.

 

Applications

ELISA and Western Blotting.


Formulation

Lyophilized at 1 mg/mL in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.

 

Storage

Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.  Avoid repeated freezing/thawing cycles.

 

Quality Control Test

BCA to determine the quantity of the protein.

SDS PAGE to determine the purity of the protein.

 

SDS-PAGE Gel

 

 

 

 

Contact Us

 

Address:

 

5/F, Biotech Centre 2, No. 11 Science Park West Avenue, 

Hong Kong Science Park, Shatin, N.T., Hong Kong

Email: 

info@immunodiagnostics.com.hk      sales@immunodiagnostics.com.hk

Phone:

(+852) 3502 2780    

Fax:  

(+852)  3502 2781

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