Home » Research Products » Recombinant Proteins » Human Histone H2A

Human Histone H2A

Product Code: 41810

 

Origin:

Recombinant

Cat. No.:

41810

Tag:

N-terminal 6xHis

Size:

0.1 mg

Source:

E.coli

Purity:

>90%

Other Names:

H2A

Species:

Human

 

 

Description

Expressed in E.coli cells with total 156 AA. Mw: 17.3 kDa (calculated). 

N-terminal 6xHis-tag and TEV cleavage site, 27 extra AA (highlighted).

Recombinant antigen for research use or manufacturing only.

 

Introduction to the Molecule

Histones are proteins that package DNA into nucleosomes and are responsible for maintaining the shape and structure of a nucleosome.

There are five families of histones known to date, H1/H5, H2A, H2B, H3, and H4. H2A, along with H2B, H3 and H4, is considered as a core histone.

Anti-histone autoantibodies are found in 50%-70% of patients with systemic lupus erythematosus (SLE) and in more than 95% of patients with drug-induced lupus erythematosus.  

 

 

Immunological Function

As an autoantigen, H2A binds with IgG-type human auto-antibodies.

 

Amino Acid Sequence

METSYYHHHH HHDYDIPTTE NLYFQGASGR GKQGGKARAK AKSRSSRAGL QFPVGRVHRL
LRKGNYSERV GAGAPVYLAA VLEYLTAEIL ELAGNAARDN KKTRIIPRHL QLAIRNDEEL
NKLLGRVTIA QGGVLPNIQA VLLPKKTESH HKAKGK

 

Applications

Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.


Formulation

Lyophilized at 1 mg/mL in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).

 

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.

 

Storage

Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.  Avoid repeated freezing/thawing cycles.

 

Quality Control Test

BCA to determine the quantity of the protein.

SDS PAGE to determine the purity of the protein.

 

SDS-PAGE Gel

 

 

 

 

Contact Us

 

Address:

 

5/F, Biotech Centre 2, No. 11 Science Park West Avenue, 

Hong Kong Science Park, Shatin, N.T., Hong Kong

Email: 

info@immunodiagnostics.com.hk      sales@immunodiagnostics.com.hk

Phone:

(+852) 3502 2780    

Fax:  

(+852)  3502 2781

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