Home » Research Products » Recombinant Proteins » Human heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP/RA33)

Human heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP/RA33)

Product Code: 41550

 

Origin:

Recombinant

Cat. No.:

41550

Tag:

N-terminal 6xHis

Size:

0.1 mg

Source:

Spodoptera frugiperda Sf9

Purity:

>90%

Other Names:

RA33,hnRPA2B1

Species:

Human

 

 

Description

Expressed in insect Sf9 cells with total 391 AA. Mw: 42 kDa (calculated). 
N-terminal 6xHis-tag and TEV cleavage site, 27 extra AA (highlighted).​

Recombinant antigen for research use or manufacturing only.

 

Introduction to the Molecule

RA33, also known as heterogeneous nuclear ribonucleoprotein A2/B1, is an autoantigen in multiple autoimmune diseases in human beings.

Anti-RA33 autoantibodies can be specifically detected in the sera of approximately 30-35% of patients with rheumatoid arthritis (RA), 35-40% of patients with mixed connective tissue disease (MCTD) and around 20-25% of patients with systemic lupus erythematosus (SLE), hence can be used as a specific immunological serum biomarker for these diseases.

 

Immunological Function

As an autoantigen, RA33 binds with IgG-type human auto-antibodies.

 

Amino Acid Sequence

METSYYHHHH HHDYDIPTTE NLYFQGAEKT LETVPLERKK REKEQFRKLF IGGLSFETTE
ESLRNYYEQW GKLTDCVVME TRDPASKRSR GFGFVTFSSM ETAEVDAAME TAARPHSIDG
RVVEPKRAVA REESGKPGAH VTVKKLFVGG IKEDTEEHHL RDYFEEYGKI DTIEIITDRQ
SGKKRGFGFV TFDDHDPVDK IVLQKYHTIN GHNAEVRKAL SRQEMETQEV QSSRSGRGGN
FGFGDSRGGG GNFGPGPGSN FRGGSDGYGS GRGFGDGYNG YGGGPGGGNF GGSPGYGGGR
GGYGGGGPGY GNQGGGYGGG YDNYGGGNYG SGNYNDFGNY NQQPSNYGPM ETKSGNFGGS
RNMETGGPYG GGNYGPGGSG GSGGYGGRSR Y

 

Applications

Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.


Formulation

Lyophilized at 1 mg/mL in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).

 

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.

 

Storage

Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.  Avoid repeated freezing/thawing cycles.

 

Quality Control Test

BCA to determine the quantity of the protein.

SDS PAGE to determine the purity of the protein.

 

SDS-PAGE Gel

 

 

 

 

Contact Us

 

Address:

 

5/F, Biotech Centre 2, No. 11 Science Park West Avenue, 

Hong Kong Science Park, Shatin, N.T., Hong Kong

Email: 

info@immunodiagnostics.com.hk      sales@immunodiagnostics.com.hk

Phone:

(+852) 3502 2780    

Fax:  

(+852)  3502 2781

 

Write a review

Your Name:


Your Review: Note: HTML is not translated!

Rating: Bad           Good

Enter the code in the box below: