Home » Research Products » Rapid human C-reactive Protein (CRP) ELISA

Rapid human C-reactive Protein (CRP) ELISA

Product Code: 31222
Cat. No.
Assay range
Rapid Human CRP ELISA
0.78 -100 ng/ml
370.00 US$


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C-reactive protein (CRP) is a circulating protein mainly secreted from the liver. This acute phase protein consists of five identical non-glycosylated subunits of 23 kDa, that give rise to a symmetrically arranged globular protein with molecular weight of approximately 120 kDa.1 It has long been recognized that CRP is closely related to immunology, inflammation and host defence, as a result it has been used as an inflammatory marker. However, the development of high-sensitivity CRP (hsCRP) ELISA had addressed its role in other clinical issues. There is accumulating evidence suggesting the important role that CRP plays in mediating cardiovascular diseases (CVD) and type 2 diabetes.2-4 Normally CRP is presenting only in a trace amount in circulation (<1 µg/ml)5-6 but can increase over 1,000-fold under acute inflammatory state. Individual with blood CRP levels <1 µg/ml, 1-3 µg/ml and >3 µg/ml is considered to have low, moderate and high risk, respectively, of CVD and myocardial infraction.7 Therefore, blood CRP level has become a promising measure of CVD risk.8-9




The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.


Human CRP standard curve (4-parameter)




A. Sensitivity:

The lowest level of human CRP that can be detected by this assay is 0.23 ng/ml.


B. Specificity:

The antibody pair used in this assay is specific to human CRP and does not cross-react with mouse or rat CRP, or other cytokine and hormone molecules including human resistin, TNFα, ANGPTL4, insulin, leptin and IL6.


C. Precision:


Intra-assay Precision (Precision within an assay)

Two samples of known concentration were tested 16 times on one plate.


Inter-assay Precision (Precision between assays)

Three samples of known concentration were tested in 10 separate assays.


D. Recovery:

Serum samples were spiked with different amounts of human CRP and assayed.





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