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Rapid human Cystatin C ELISA

Product Code: 31241
Cat. No.
Assay range
Rapid Human Cystatin C ELISA
0.23 -30 ng/ml
330.00 US$


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Human cystatin c (or cystatin 3), which is composed of 120 amino acid residues, belongs to the cystatins superfamilly that inactivates lysosomal cysteine proteinases. As a strongly cationic and low-molecular weight (13.4 kDa) protein, it is almost freely filtered across the glomerular membrane, and is mainly used as a biomarker of kidney function. A growing body of evidence suggests that cystatin c is a more reliable biomarker of glomerular filtration rate than creatinine [1-3] . In addition to kidney disease, altered serum levels of cystatin c are associated with several types of cardiovascular disease, including myocardial infarction, stroke, heart failure, peripheral arterial disease and metabolic syndrome [4-7] . It also seems to play a role in brain disorders involving amyloid, such as Alzheimer's disease [8, 9] . Furthermore, Cystatin c has also been investigated as a prognostic marker in several forms of cancer [11, 12] .




The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.


Human cystatin c standard curve (4-parameter)




A. Sensitivity:

The lowest level of human cystatin c that can be detected by this assay is 0.23 ng/ml.


B. Specificity:

The antibodies used in this assay are specific to human cystatin c and do not cross-react with mouse and rat cystatin c, and other cytokine or hormone molecules.


C. Precision:


Intra-assay Precision (Precision within an assay)

Three samples of known concentration were tested 12 times on one plate.


Inter-assay Precision (Precision between assays)

Three samples of known concentration were tested in 10 separate assays.


 D. Recovery:

Serum samples were spiked with different amounts of human cystatin c and assayed.





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