Home » Research Products » Human plasminogen activator inhibitor type-1 protein (PAI-1) ELISA

Human plasminogen activator inhibitor type-1 protein (PAI-1) ELISA

Product Code: 31070
Cat. No.
Assay range
Human PAI-1 ELISA kit
0.016-2 ng/ml
440 US$


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Plasminogen activator inhibitor-1 (PAI-1) is the primary inhibitor of tissue-type and urokinase-type plasminogen activator, playing a major role in fibrinolysis1-2 . PAI-1 is mainly produced by the endothelium, but is also secreted by other tissue types, such as adipose tissue3 . It is normally present at low levels in plasma and tissue, but its expression and release increased in various disease states (such as a number of forms of cancer), as well as in obesity and the metabolic syndrome4 . PAI-1 is also involved in the pathophysiology of renal, pulmonary, cardiovascular, and metabolic diseases 5-8 . Elevated local or systemic PAI-1 can also exacerbate such pathologic conditions.




The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.


Human PAI-1 standard curve (4-parameter)




A. Sensitivity:

The lowest level of PAI-1 that can be measured by this assay is 0.031 ng/ml.


B. Specificity:

The antibodies used in this assay are specific to human PAI-1 and do not cross-react with mouse and rat PAI-1, and other cytokine or hormone molecules including human resistin, TNFα, ANGPTL4, insulin, leptin and IL6.


C. Precision:

Intra-assay Precision (Precision within an assay)

Two samples of known concentration were tested 16 times on one plate


Inter-assay Precision (Precision between assays)

Four samples of known concentration were tested in 8 separate assays.


D. Recovery:

Serum samples were spiked with different amounts of human PAI-1 and assayed.





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