Mouse FGF-21 ELISA
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FGF21 is a metabolic hormone mainly produced in liver. It regulates glucose and lipid metabolism through pleiotropic actions in multiple tissues, and protects against metabolic damages under various stresses.
In humans, high circulating FGF21 levels are found in obesity and multiple cardiometabolic disorders, including metabolic syndrome, type 2 diabetes, non-alcoholic fatty liver disease and coronary artery disease. Serum FGF21 is a potential biomarker for the early detection and risk prediction of these diseases.
TYPICAL STANDARD CURVE
The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.
Mouse FGF-21 standard curve (4-parameter)
The lowest level of mouse FGF-21 that can be detected by this assay is 15.6 pg/ml.
Cross reactivity of recombinant proteins
Intra-assay Precision (Precision within an assay) C.V <8%.
Inter-assay Precision (Precision between assays) C.V <10%.
The recovery of the assay was determined by adding various amounts FGF21 to a sample. The measured concentration of the spiked sample in the assay was compared to the expected concentration. The average recovery was 92%.
To assess the linearity of the assay, a sample with high level of FGF-21 was serially diluted with the 1×Assay buffer to produce samples with values within the dynamic range of the assay.
Publications Citing Our Products:
Ye D, Wang Y, Li H, et al. Hepatology. 2014; 60(3): 977-989.
Liang Q, Zhong L, Zhang J, et al. Diabetes. 2014; 63(12): 4064-4075.
Li H, Gao Z, Zhang J, et al. Diabetes. 2012; 61(4): 797-806.
So, Wing Yan, et al. Diabetes. 2013; 62(11):3751-9.
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