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Human Galectin-3 ELISA

Product Code: 31690

 

Cat. No.
Product
Assay range
Size
Price*
31690
Human Galectin-3 ELISA kit
0.625-40 ng/ml
96T/Kit
400.00 US$

 

 *Shipping cost is not included, please provide the payable FedEx account while autocreating purchase orders.

 

 

INTRODUCTION

 

Galectins are a family of animal lectins with carbohydrate-binding activity and specificity for N-acetyllactosamine (LacNac). [1] Galectin-3 was the unique chimera type lectins. [2] Galectin-3 lacks a signal peptide for classical secretion but presents and active inside and outside the cell. Human galectin-3, 29-36 KDa, exhibits numerous autocrine and paracrine effects and mediates in cell adhesion, cell activation and apoptosis and up- or down-regulation in cancer [3]. In addition, plays an important role in immune response and inflammation. While the cytosolic galectin-3 involves in the cell proliferation, differentiation and survival.
Serum levels of galectin-3 elevated in Behcet’s Diseases[4], Thyroid disease[5], Alzheimer’s disease[6], cardiovascular disease such as LAA stroke[7] and in serval cancers especially when they are metastatic[8]. Moreover, circulating levels of galectin-3 are higher in obese humans and indicative for a role in insulin resistance in man.[9]

 

TYPICAL STANDARD CURVE

 

The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.

 

 

ASSAY CHARACTERISTICS

 

A. Sensitivity:

 

The lowest level of human Galectin-3 that can be measured by this assay is 0.145 ng/ml.

 

B. Specificity:

 

C. Precision:

 

 

D. Spiking

 

Serum samples were assayed by adding 90 μl of sample and 10 μl of spike stock solution calculated to yield the intended 0, 2.5, 20 or 40 ng/ml spike concentration

 

E. Linearity

 

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human galectin-3 were serially diluted with the 1×Assay buffer to produce samples with values within the dynamic range of the assay.

 

F. Validation

 

Serum galectin-3 levels were measured in three different groups, women with normal glucose tolerance during pregnancy (NGT), gestational diabetes mellitus (GDM) and healthy women (age 20-35,Healthy) respectively.

 

Reference:

 

1. Dumic, Jerka, Sanja Dabelic, and Mirna Flögel. "Galectin-3: an open-ended story." Biochimica et Biophysica Acta (BBA)-General Subjects 1760.4 (2006): 616-635.
2. Krześlak, Anna, and Anna Lipińska. "Galectin-3 as a multifunctional protein." Cell Mol Biol Lett 9.2 (2004): 305-28.
3. van den Brûle, Frédéric, Stèphane Califice, and Vincent Castronovo. "Expression of galectins in cancer: a critical review." Glycoconjugate journal 19.7 (2002): 537-542.
4. Lee, Y. J., et al. "Serum galectin-3 and galectin-3 binding protein levels in Behçet's disease and their association with disease activity." Clinical and experimental rheumatology 25.4 Suppl 45 (2007): S41-5.
5. Saussez, Sven, et al. "Serum galectin-1 and galectin-3 levels in benign and malignant nodular thyroid disease." Thyroid 18.7 (2008): 705-712.
6. Wang, Xuexin, et al. "Elevated Galectin-3 levels in the serum of patients with Alzheimer's disease." American Journal of Alzheimer's Disease & Other Dementias® 30.8 (2015): 729-732.
7. He, Xin-Wei, et al. "Serum levels of galectin-1, galectin-3, and galectin-9 are associated with large artery atherosclerotic stroke." Scientific reports 7 (2017).
8. Nangia-Makker, Pratima, et al. "Galectin-3 cleavage: a novel surrogate marker for matrix metalloproteinase activity in growing breast cancers." Cancer research 67.24 (2007): 11760-11768.
9. Li, Pingping, et al. "Hematopoietic-derived galectin-3 causes cellular and systemic insulin resistance." Cell 167.4 (2016): 973-984
 

 

 

 

 

 

 

 

 

Contact Us

 

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Hong Kong Science Park, Shatin, N.T., Hong Kong

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(+852) 3502 2780

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(+852)  3502 2781

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