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Human TSP-2 ELISA kit

Product Code: 31101
Cat. No.
Product
Assay range
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Price*
31101
 
Human Thrombospondin-2 (TSP-2) Elisa kit
0.156 -10 ng/ml
Sample size: 100 ul
96T/Kit
 

 

 

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INTRODUCTION

 

Thrombospondin-2 (TSP-2) is a 150 kDa calcium-binding protein.  TSP-2 inhibits angiogenesis through direct effect on endothelial cell migration, proliferation, survival and apoptosis [1].  It is a potential risk factor in heart disease [2].

 

PRINCIPLE OF THE ASSAY

This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human TSP-2. Standards and samples are pipetted into the wells and any human TSP-2 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human TSP-2 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human TSP-2 bound initially.  The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human TSP-2, the unknown sample concentration can be interpolated from a reference curve included in each assay.

 

TYPICAL STANDARD CURVE

 

The following standard curve is provided for demonstration only. A standard curve should be generated for each assay.

 

Insulin standard curve (log-log)

 

ASSAY CHARACTERISTICS

 

A. Sensitivity

The lowest insulin level that can be measured by this assay is 0.156 ng/ml.

 

B. Precision

Intra-assay Precision (Precision within an assay) C.V <10%.

Inter-assay Precision (Precision between assays) C.V <10%.

 

Publications Citing Our Products:

 

[1] Patrick R. L., et al. (2012)  Cold Spring Harb Perspect Med 2: a 00627.
[2] Morikawa N., et al. (2019)  Int Heart J 20;60(2):310-317

 

 

 

 

Contact Us

 

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5/F, Biotech Centre 2, No. 11 Science Park West Avenue,

Hong Kong Science Park, Shatin, N.T., Hong Kong

Email:

info@immunodiagnostics.com.hk      sales@immunodiagnostics.com.hk

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(+852) 3502 2780

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(+852)  3502 2781

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