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Recombinant Adiponectin with His Tag (Mouse)

Product Code: 42010

Type:

Recombinant

Cat. No.:

42010

Tag:

His

Size:

0.1  mg

Source:

E.Coli

Purity:

>90%

Other names:

Acrp30, GBP28, AdipoQ

Species:

Mouse

 

For bulk order or without FedEx acc, please contact us by email
Payable FedEx acc:

 

Introduction

 

Adiponectin is an adipokine exclusively expressed in adipose tissues with potent anti-diabetic, anti-atherogenic and anti-inflammatory functions.
In humans, decreased serum adiponectin levels are associated with increased body mass index (BMI), decreased insulin sensitivity, less favourable plasma lipid profiles, increased inflammation and increased risk for the development of type 2 diabetes, hypertension and coronary heart diseases.

 

Description

Total 258 AA. Mw: 28.3 kDa (calculated). N-terminal His-tag and TEV cleavage site,

28 extra AA (highlighted).

 

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSEDDVTTTEELAPALVPPPKGTCAGWMAGIPGHPGHNG

TPGRDGRDGTPGEKGEKGDAGLLGPKGETGDVGMTGAEGPRGFPGTPGRKGEPGEAAYMYRSAFSVG

LETRVTVPNVPIRFTKIFYNQQNHYDGSTGKFYCNIPGLYYFSYHITVYMKDVKVSLFKKDKAVLFTYDQY

QEKNVDQASGSVLLHLEVGDQVWLQVYGDGDHNGLYADNVNDSTFTGFLLYHDTN

 

Formulation

Lyophilized in 1 mg/mL in PBS.

 

 

Endotoxin Level

<0.2 EU/ug.

 

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the

lyophilized pellet dissolve completely.

 

Storage

Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.

Avoid repeated freezing /thawing cycles.

 

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

 

Applications

Cell culture and/or animal studies, ELISA, Western blotting

 

SDS-PAGE Gel

 

Publications Citing Our Products:

 

 Yau S Y, Li A, Hoo R L C, et al. Proc Natl Acad Sci U S A.  2014; 111(44): 15810-15815.

 

 

 

 

Contact Us

 

Address:

 

5/F, Biotech Centre 2, No. 11 Science Park West Avenue,

Hong Kong Science Park, Shatin, N.T., Hong Kong

Email:

info@immunodiagnostics.com.hk      sales@immunodiagnostics.com.hk

Phone:

(+852) 3502 2780

Fax:

(+852)  3502 2781

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