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Mouse insulin, Ultra sensitive (ELISA)

Product Code: 32380
Cat. No.
Product
Assay range
Size
Price*
32380
 
Ultra sensitive mouse Insulin Elisa kit
0.013-1.5ng/ml
Sample size: 25 ul
96T/Kit
 
430.00 US$
 

 

*Shipping cost is not included, please provide the payable FedEx account while autocreating purchase orders.

Payable FedEx acc:

 For bulk order or without FedEx account, please contact us by email: sales@immunodiagnostics.com.hk

 

 

INTRODUCTION

 

Insulin is a peptide hormone exclusively produced in pancreatic beta-cells. It consists of A chain and B chain, which are linked by two disulfide bridges.
Insulin is the primary hormone responsible for glucose metabolism. Impaired insulin secretion and insulin resistance are key causes of type 2 diabetes (T2D).

 

PRINCIPLE OF THE ASSAY

 

This assay is a two-site ELISA. The micro-plate is pre-coated with a monoclonal antibody against insulin. Standards and samples are added into the wells and coincubated with a monoclonal antibody conjugated to horseradish peroxidase (HRP) enzyme. After wash step to remove any unbound substances, TMB substrate is added and colour develops in proportion to the amount of insulin bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured insulin, the unknown sample concentration can be interpolated from a reference curve included in each assay.

 

TYPICAL STANDARD CURVE

 

The following standard curve is provided for demonstration only. A standard curve should be generated for each assay.

 

dard curve (log-log)

 

ASSAY CHARACTERISTICS

 

A. Sensitivity

The lowest insulin level that can be measured by this assay is 0.013 ng/ml.

 

B. Precision

Intra-assay Precision (Precision within an assay) C.V. < 10%.

Inter-assay Precision (Precision between assays) C.V. <10%.

 

C. Recovery

The recovery of the assay was determined by adding various amounts insulin to a sample. The measured concentration of the spiked sample in the assay was compared to the expected concentration. The average recovery was 92%.

 

D. Specificity

Percent of cross reactivity

Human insulin 100%

Rat insulin 100%

 

E. Validation

Insulin levels were measured at various time points after intra-peritoneal glucose challenge in overnight fasting C57 mice (standard chow fed).

 

Publications Citing Our Products:

 

 Cheng K.K, Lam KS, et al. Proc Natl Acad Sci U S A. 2012; 109(23):8919-24.
 Cheng K.K, et al. Diabetes. 2014;  63(11): 3748-58.
 Shu L, Hoo R L C, Wu X, et al. Nat Commun. 2017; 8:14147.
 Li X, Cheng K. K, Liu Z, et al. Nat Commun. 2016; 7:11740.
 Lin Z, Tian H, et al. Cell Metab. 2013; 17(5):779-89.
 Liang Q, Zhong L, Zhang J, et al. Diabetes. 2014; 63(12): 4064-4075.
 Cheng, Sam Tsz Wai, et al. PLoS One. 2016; 11(1):e0147391.
 So, W. Y., et al. Cell Death Dis. 2015; 6:e1707.
 Wang, Lin, et al. PLoS One. 2015; 10(6):e0128216.
 

 

 

Contact Us

 

Address:

 

5/F, Biotech Centre 2, No. 11 Science Park West Avenue,

Hong Kong Science Park, Shatin, N.T., Hong Kong

Email:

info@immunodiagnostics.com.hk      sales@immunodiagnostics.com.hk

Phone:

(+852) 3502 2780

Fax:

(+852)  3502 2781

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