Home » Search » Mouse IL-33 ELISA kit

Mouse IL-33 ELISA kit

Product Code: 32750
Cat. No.
Product
Assay range        
Size       
Price*
 32750
 Mouse IL-33 ELISA kit
31.25-2000 pg/ml
96T/Kit
400.00 US$

 

*Shipping cost is not included, please provide the payable FedEx account while auto-creating purchase orders.

 
Payable FedEx acc:
 

 For bulk order or without FedEx account, please contact us by email: sales@immunodiagnostics.com.hk

 

 

TYPICAL STANDARD CURVE

 

The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.

 

Human adiponectin standard curve (4-parameter)

 

ASSAY CHARACTERISTICS

 

A. Sensitivity:

The lowest level of human adiponectin that can be detected by this assay is 6.5 pg/ml.

 

B. Precision:

Intra-Assay Precision (Precision within an assay) 
Three samples of known concentration were tested 8 times on one plate.

Intra-Assay Precision (Precision within an assay) 
Three samples of known concentration were tested 8 times on separate plates.

 

C. Spiking:

Cell culture supernate samples were assayed by adding 90 µl of the sample and 10 µl of spike stock solution calculated to yield the intended 0, 150, 750 or 1500 pg/ml spike concentration.

 

D Linearity:

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-33 were serially diluted with the 1×Assay buffer to produce samples with values within the dynamic range of the assay.

 

F. Validation:

Cell culture supernates:
Lungs from mice were chopped into 1-2 mm pieces and cultured in 15 mL RPMI supplemented with 10% FBS, 50 μM β-ME, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate or stimulated with 1.0 μg/mL lipopolysaccharide (LPS) for 24 hours. Cell culture medium was removed and assayed for levels of mouse IL-33. In addition, we also collected the cell lysates and measured IL-33 levels and compared with the total protein levels in the cell lysates.

A per-body-weight dose (100 ug/10g) LPS were injected into mice (i.p.) and after 12 hours mice were sacrificed. Lungs and spleens from mice were chopped into 1-2 mm pieces and cultured in 6-well plate. After 24 hours incubation, the cell culture medium was removed and assayed for levels of mouse IL-33.

 

 

 

 

 

Contact Us

 

Address:

 

5/F, Biotech Centre 2, No. 11 Science Park West Avenue,

Hong Kong Science Park, Shatin, N.T., Hong Kong

Email:

info@immunodiagnostics.com.hk      sales@immunodiagnostics.com.hk

Phone:

(+852) 3502 2780

Fax:

(+852)  3502 2781

Write a review

Your Name:


Your Review: Note: HTML is not translated!

Rating: Bad           Good

Enter the code in the box below: