Home » Search » Human Small Nuclear Ribonucleoprotein Polypeptide A (snRNPA)

Human Small Nuclear Ribonucleoprotein Polypeptide A (snRNPA)

Product Code: 41510

Origin:

Recombinant

Cat. No.:

41510

Tag:

N-terminal 6xHis

Size:

0.1 mg

Source:

Spodoptera frugiperda Sf9

Purity:

>90%

Other Names:

U1-snRNPA, RNPA

Species:

Human

 

Description

Expressed in insect Sf9 cells with total 306 AA. Mw: 34.2 KDa (calculated).

N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).

Recombinant antigen for research use or manufacturing only.

 

Introduction to the Molecule

Small nuclear ribonucleoprotein complexes (abbreviated as U-snRNP) are essential for splicing of precursor

mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus and consists of one small

uridylate-rich RNA (U1 RNA) complexed with several proteins, and the three 68/70 kDa (snRNP68/70),

A polypeptides (snRNPA) and C polypeptides (snRNPC) are unique to the U1-snRNP particle.

Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease (MCTD)

and 30% of patients with SLE.

 

Immunological Function

As an autoantigen, RNP-A binds with IgG-type human auto-antibodies.

 

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAAVPETRPNHTIYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSL

KMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQYAKTDSDIIAKMKGTFVERDRKREKRKPKSQETPATK

KAVQGGGATPVVGAVQGPVPGMPPMTQAPRIMHHMPGQPPYMPPPGMIPPPGLAPGQIPPGAMPPQQL

MPGQMPPAQPLSENPPNHILFLTNLPEETNELMLSMLFNQFPGFKEVRLVPGRHDIAFVEFDNEVQAGAAR

DALQGFKITQNNAMKISFAKK

 

Applications

Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

 

Formulation

Lyophilized at 1 mg/mL in storage buffer (20mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).

 

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized

pellet dissolve completely.

 

Storage

Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.  Avoid repeated

freezing /thawing cycles.

 

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

 

SDS-PAGE Gel

 

 

 

 

Contact Us

 

Address:

 

5/F, Biotech Centre 2, No. 11 Science Park West Avenue, 

Hong Kong Science Park, Shatin, N.T., Hong Kong

Email: 

info@immunodiagnostics.com.hk      sales@immunodiagnostics.com.hk

Phone:

(+852) 3502 2780    

Fax:  

(+852)  3502 2781

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Tags: snRNPA