Home » Search » Human Small Ribonucleoprotein Associated Protein B B' (SmB/B’)

Human Small Ribonucleoprotein Associated Protein B B' (SmB/B’)

Product Code: 41530

 

Origin:

Recombinant

Cat. No.:

41530

Tag:

N-terminal 6xHis

Size:

0.1 mg

Source:

Spodoptera frugiperda Sf9

Purity:

>90%

Other Names:

SmB/B’, snRNPB/B’

Species:

Human

 

Description

Expressed in insect Sf9 cells with total 265 AA. Mw: 27.6 KDa (calculated).

N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).

Recombinant antigen for research use or manufacturing only.

 

Introduction to the Molecule

Small nuclear ribonucleoprotein complexes (abbreviated as U-snRNP) are essential for splicing

of precursor mRNA molecules and snRNPB is one of several nuclear proteins which are found in

common among U1, U2, U4/U6, and U5 small ribonucleoprotein particles (snRNPs).

Autoantibodies from patients with systemic lupus erythematosus (SLE) recognize epitopes on snRNPB/B’.

 

Immunological Function

As an autoantigen, SmB/B’ binds with IgG-type human auto-antibodies.

 

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGATVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDC

DEFRKIKPKNSKQAEREEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGIGRAAGRGIPAG

VPMPQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAAAATASIAGAPTQYPPGRGGPPPPMGRGAP

PPGMMGPPPGMRPPMGPPMGIPPGRGTPMGMPPPGMRPPPPGMRGPPPPGMRPPRP.

 

Applications

Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

 

Formulation

Lyophilized at 1 mg/mL in storage buffer (20mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).

 

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the

lyophilized pellet dissolve completely.

 

Storage

Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.  Avoid repeated

freezing /thawing cycles.

 

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

 

SDS-PAGE Gel

 

 

 

 

Contact Us

 

Address:

 

5/F, Biotech Centre 2, No. 11 Science Park West Avenue, 

Hong Kong Science Park, Shatin, N.T., Hong Kong

Email: 

info@immunodiagnostics.com.hk      sales@immunodiagnostics.com.hk

Phone:

(+852) 3502 2780    

Fax:  

(+852)  3502 2781

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Tags: SmB/B'