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Human Centromere Protein A (CENPA)

Product Code: 41610

Origin:

Recombinant

Cat. No.:

41610

Tag:

N-terminal 6xHis

Size:

0.1 mg

Source:

Spodoptera frugiperda Sf9

Purity:

>95%

Other Names:

CENPA

Species:

Human

 

Description

Expressed in insect Sf9 cells with total 164 AA. Mw: 19.0 KDa (calculated).

N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).

Recombinant antigen for research use or manufacturing only.

 

Introduction to the Molecule

Centromere proteins are a group of proteins which form and/or mediate the function of

centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach

and pull the chromosomes apart in cell division. The centromere protein A (CENPA) is one of

them. CENPA contains a histone H3 related histone fold domain and can be incorporated into

centromeric chromatin due to its histone-like properties. Anti-CENPA autoantibodies are an

important marker for diagnosis of Scleroderma / CREST syndrome.

 

Immunological Function

As an autoantigen, CENP-A binds with IgG-type human auto-antibodies.

 

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAGPRRRSRKPEAPRRRSPSPTPTPGPSRRGPSLGASSHQHSRRR

QGWLKEIRKLQKSTHLLIRKLPFSRLAREICVKFTRGVDFNWQAQALLALQEAAEAFLVHLFEDAYLLTLHA

GRVTLFPKDVQLARRIRGLEEGLG

 

Applications

Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

 

Formulation

Lyophilized at 1 mg/mL in storage buffer (20mM Tris, 150mM NaCl, 8M Urea, PH8.0).

 

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the

lyophilized pellet dissolve completely.

 

Storage

Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.  Avoid

repeated freezing /thawing cycles.

 

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

 

SDS-PAGE Gel

 

 

 

 

Contact Us

 

Address:

 

5/F, Biotech Centre 2, No. 11 Science Park West Avenue, 

Hong Kong Science Park, Shatin, N.T., Hong Kong

Email: 

info@immunodiagnostics.com.hk      sales@immunodiagnostics.com.hk

Phone:

(+852) 3502 2780    

Fax:  

(+852)  3502 2781

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Tags: CENPA