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BCOADC-E2 (Human Branched Chain 2-oxo-acid Dehydrogenase Complex E2)

BCOADC-E2 (Human Branched Chain 2-oxo-acid Dehydrogenase Complex E2)

SKU: 41A062

★ Download Datasheet PDF ★

 

 

Origin:

Recombinant

Cat. No.:

41A062

Tag:

N-terminal 6xHis

Size:

0.1 mg

Source:

Spodopterafrugiperda Sf9

Purity:

>90%

Other names:

BCOADC-E2, BCKAD-E2,BCATE2

Species:

Human

  • INTRODUCTION

    BCOADC-E2 is the central E2 component of branched chain 2-oxo acid dehydrogenase complex (BCOADC), which is a mitochondrial multienzyme complex involved in the maintenance of the cellular redox state. 

     

  • DESCRIPTION

    Expressed in insect Sf9 cells with a total 446 AA.

    Mw: 49.5 KDa(calculated).

    N-terminal 6xHis-tag and TEV cleavage site, extra 25 AA (highlighted).

    Recombinant antigen for research use or manufacturing only.

  • AMINO ACID SEQUENCE

    MSYYHHHHHHDYDIPTTENLYFQGAGQVVQFKLSDIGEGIREVTVKEWYVKEGDTVSQFDSICEVQSDKASVTITSRYDGVIKKLYYNLDDIAYVGKPLVDIETEALKDSEEDVVETPAVSHDEHTHQEIKGRKTLATPAVRRLAMENNIKLSEVVGSGKDGRILKEDILNYLEKQTGAILPPSPKVEIMPPPPKPKDMTVPILVSKPPVFTGKDKTEPIKGFQKAMVKTMSAALKIPHFGYCDEIDLTELVKLREELKPIAFARGIKLSFMPFFLKAASLGLLQFPILNASVDENCQNITYKASHNIGIAMDTEQGLIVPNVKNVQICSIFDIATELNRLQKLGSVSQLSTTDLTGGTFTLSNIGSIGGTFAKPVIMPPEVAIGALGSIKAIPRFNQKGEVYKAQIMNVSWSADHRVIDGATMSRFSNLWKSYLENPAFMLLDLK

  • STORAGE

    Store at –80°C.  Avoid repeated freezing/thawing cycles.

  • QUALITY CONTROL TEST

    BCA to determine quantity of the protein.

    SDS PAGE to determine purity of the protein.

  • FORMULATION

    Liquid in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, Protease inhibitor, pH8.0).

  • APPLICATION

    Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

  • IMMUNOLOGICAL FUNCTION

    As an autoantigen, BCOADC-E2 binds with IgG-type human auto-antibodies.

$374.00Price

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