CENP-A (Human Centromere Protein A)
Origin:
Recombinant
Cat. No.:
41610
Tag:
N-terminal 6xHis
Size:
0.1 mg
Source:
Spodoptera frugiperda Sf9
Purity:
>95%
Other Names:
CENP-A, CENP A
Species:
Human
INTRODUCTION
Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. The centromere protein A (CENPA) is one of them. CENPA contains a histone H3 related histone fold domain and can be incorporated into centromeric chromatin due to its histone-like properties. Anti-CENPA autoantibodies are an important marker for diagnosis of Scleroderma / CREST syndrome.
IMMUNOLOGICAL FUNCTION
As an autoantigen, CENP-A binds with IgG-type human auto-antibodies.
AMINO ACID SEQUENCE
MSYYHHHHHHDYDIPTTENLYFQGAGPRRRSRKPEAPRRRSPSPTPTPGPSRRGPSLGASSHQHSRRRQGWLKEIRKLQKSTHLLIRKLPFSRLAREICVKFTRGVDFNWQAQALLALQEAAEAFLVHLFEDAYLLTLHAGRVTLFPKDVQLARRIRGLEEGLG
APPLICATIONS
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
FORMULATION
Liquid in 8M Urea buffer (pH 8.0).
DESCRIPTION
Expressed in insect Sf9 cells with a total of 164 AA.
Mw: 19.0 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
STORAGE
Store at –80°C. Avoid repeated freezing/thawing cycles
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.