FGF-1/FGF-Acidic, Human
Species: | Human |
Expression System: | E.coli |
MW: | 17 kDa (reducing) |
Accession: | P05230-1 |
Cat No. | UA040032 |
Endotoxin: | ＜0.1 EU/μg |
Purity: | >95% as determined by SDS-PAGE |
INTRODUCTION
Human fibroblast growth factor 1 (FGF-1), also known as acidic fibroblast growth factor (FGF-Acidic), is one of the best characterized members of the FGF superfamily. FGF-1 is a powerful mitogen that exhibits potent effects on numerous different types of cells. It plays a number of roles in several important physiological and pathological processes, such as embryonic development, morphogenesis, angiogenesis, wound healing and atheromasias, carcinogenesis, development, and invasion of cancer. FGF-1 is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The association of FGF-1 with heparan sulfate is a
prerequisite for its subsequent interaction with FGF receptors. Ligation triggers receptor dimerization, transphosphorylation, and internalization of receptor/FGF complexes.Amino Acid Sequence
Phe16-Asp155
MFNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSDPhysical Appearance:
Lyophilized powder
Buffer:
20mM PB, 150mM NaCl, 1mM DTT, pH7.4
Reconstitution:
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- 1 week, 2 to 8 °C under sterile conditions after reconstitution.
Note: Please avoid repeated freeze-thaw cycles