Human Galectin-3 (Gal-3)
Origin: | Recombinant | Cat. No.: | 41690 |
Tag: | N-terminal 6xHis | Size: | 0.1 mg |
Source: | E.coli | Purity: | >95% |
Other names: | Gal-3，MAC-2, CBP 35, lectinL-29, AGER 3 | Species: | Human |
INTRODUCTION
Galectin-3 is expressed by a wide range of cell types including activated T cells, tumor cells, macrophages, osteoclasts, fibroblasts, and epithelial cells.
Galectin-3 has specific binding affinity for beta-galactoside sugar moieties and has functional roles during development, innate immunity, cell apoptosis, and tumor metastasis. Galectin-3 is associated with cancer, heart failure, stroke, and inflammation.
DESCRIPTION
Expressed in E.coli with total 293AA. Mw: 31.1 KDa (calculated).
N-terminal 6xHis-tag, EK recognition site and TEV cleavage site, 44 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
AMINO ACID SEQUENCE
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSENLYFQGAADNFSLHDALSGSGNPNPQGWPGAWGNQPAGAGGYPGASYPGAYPGQAPPGAYPGQAPPGAYPGAPGAYPGAPAPGVYPGPPSGPGAYPSSGQPSATGAYPATGPYGAPAGPLIVPYNLPLPGGVVPRMLITILGTVKPNANRIALDFQRGNDVAFHFNPRFNENNRRVIVCNTKLDNNWGREERQSVFPFESGKPFKIQVLVEPDHFKVAVNDAHLLQYNHRVKKLNEISKLGISGDIDLTSASYTMI
STORAGE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeated freezing/thawing cycles.
RECONSTITUTION
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
FORMULATION
Lyophilized at 1 mg/mL in PBS.
APPLICATION
Standard ELISA test, Western Blot.
ENDOTOXIN LEVEL
<0.01 EU per 1 μg of the protein by the LAL method.