Human Nuclear Pore Membrane Glycoprotein 210 (gp210)
Origin:
Recombinant
Cat. No.:
41A280
Tag:
N-terminal 6xHis
Size:
0.1 mg
Source:
E.coli
Purity:
>80%
Other Names:
Gp210, Nup210
Species:
Human
INTRODUCTION
The nuclear pore complex(NPC) is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. As a part of NPC, gp210 is detected with a prevalence of 26% in PBC and 4% in AIH.
DESCRIPTION
Expressed in E.coli with total 238 AA. Mw: 24 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 44 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
AMINO ACID SEQUENCE
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSENLYFQGATVCTPRDLAVPAALTPRASPGHSPHYFAASSPTSPNALPPARKASPPSGLWSPAYASHGGGGSGGGGSTVCTPRDLAVPAALTPRASPGHSPHYFAASSPTSPNALPPARKASPPSGLWSPAYASHGGGGSGGGGSTVCTPRDLAVPAALTPRASPGHSPHYFAASSPTSPNALPPARKASPPSGLWSPAYASH
STORAGE
Store at –20°C. Avoid repeated freezing/thawing cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Immunodot analysis to determine functionality of protein.
FORMULATION
Liquid in storage buffer (8M Urea, 10mM Tris, 50mM Na2HPO4, pH8.0).
APPLICATION
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
IMMUNOLOGICAL FUNCTION
As an autoantigen, gp210 binds with IgG-type human auto-antibodies.