P0 (Human Ribosomal Phosphoprotein P0)
Origin:
Recombinant
Cat. No.:
41560
Tag:
N-terminal 6xHis
Size:
0.1 mg
Source:
E.coli
Purity:
>90%
Other names:
P0, RPLP0
Species:
Human
INTRODUCTION
The ribosomal phosphoproteins (P protein antigens) are associated with the large ribosomal subunit and therefore are antigenic targets with a cytoplasmic localization. Three P proteins have been described: P0 with a molecular weight of 36 kDa, P1 (19 kDa) and P2 (17 kDa).
Autoantibodies against ribosomal P proteins are present in 10 % of SLE patients.
IMMUNOLOGICAL FUNCTION
As an autoantigen, P0 binds with IgG-type human auto-antibodies.
AMINO ACID SEQUENCE
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSENLYFQGAPREDRATWKSNYFLKIIQLLDDYPKCFIVGADNVGSKQMQQIRMSLRGKAVVLMGKNTMMRKAIRGHLENNPALEKLLPHIRGNVGFVFTKEDLTEIRDMLLANKVPAAARAGAIAPCEVTVPAQNTGLGPEKTSFFQALGITTKISRGTIEILSDVQLIKTGDKVGASEATLLNMLNISPFSFGLVIQQVFDNGSIYNPEVLDITEETLHSRFLEGVRNVASVCLQIGYPTVASVPHSIINGYKRVLALSVETDYTFPLAEKVKAFLADPSAFVAAAPVAAATTAAPAAAAAPAKVEAKEESEESDEDMGFGLFD
APPLICATIONS
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
FORMULATION
Liquid in PBS(10mM Na2HPO4, 2mM KH2PO4, 2.7mM KCl,137mM NaCl, Protease inhibitor, pH7.4).
DESCRIPTION
Expressed in E.coli with total 360 AA.
Mw: 39.2 kDa (calculated).
N-terminal 6xHis-tag, Xpress Epitope, EK recognition site and TEV cleavage site, 44 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
STORAGE
Store at –80°C. Avoid repeated freezing/thawing cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.