Human Serum Amyloid A1
Origin: | Recombinant | Cat. No.: | 41910 |
Tag: | N-terminal 6xHis | Size: | 0.1 mg |
Source: | E.coli | Purity: | >90% |
Other names: | SAA | Species: | Human |
INTRODUCTION
SAA is synthesized in the liver and secreted to the blood. When in the blood, SAA proteins form complexes with high density lipoproteins (HDL). It has been suggested that SAA is involved in the recycling of cholesterol from damaged tissues. Similar to C-reactive protein, SAA is a major acute phase protein in human being, it can be used in diagnosis, predicting outcomes and assessing the efficacy of treatment in patients with inflammation. SAA is a sensitive biomarker of acute renal allograft rejection and it can be used to monitor SAA in kidney transplant patients for the early detection of acute rejection episodes. In patients with myocardial infarction, SAA concentration is elevated to extremely high values and correlates with postinfarction complications and the mortality rate.
DATA SHEET
DESCRIPTION
Expressed in E.coli with total 146 AA. Mw: 16.6 KDa (calculated).
N-terminal 6xHis-tag, EK and TEV cleavage site, 42 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
AMINO ACID SEQUENCE
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSENLYFQRSFFSFLGEAFDGARDMWRAYSDMREANYIGSDKYFHARGNYDAAKRGPGGAWAAEVISDARENIQRFFGHGAEDSLADQAANEWGRSGKDPNHFRPAGLPEKY
STORAGE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeated freezing /thawing cycles.
RECONSTITUTION
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
FORMULATION
Lyophilized at 1 mg/mL in PBS.
APPLICATION
Standard ELISA test, Western Blot.
SDS-PAGE Gel
See image