Human Sp100
Origin:
Recombinant
Cat. No.:
41A162
Tag:
N-terminal 6xHis
Size:
0.1 mg
Source:
Spodopterafrugiperda Sf9
Purity:
>90%
Other names:
Sp100
Species:
Human
INTRODUCTION
Together with PML, Sp100 is a major constituent of the PML bodies, a subnuclear
organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis.
Autoantibody against Sp100 are suggested as asignificant role in the diagnosis of primary biliary cirrhosis.
DESCRIPTION
Expressed in insect Sf9 cells with total 903 AA.
Mw: 103.4 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, extra 25 AA (highlighted).
Recombinant antigen for research use or manufacturing only.
AMINO ACID SEQUENCE
MSYYHHHHHHDYDIPTTENLYFQGAAGGGGDLSTRRLNECISPVANEMNHLPAHSHDLQRMFTEDQGVDDRLLYDIVFKHFKRNKVEISNAIKKTFPFLEGLRDRDLITNKMFEDSQDSCRNLVPVQRVVYNVLSELEKTFNLPVLEALFSDVNMQEYPDLIHIYKGFENVIHDKLPLQESEEEEREERSGLQLSLEQGTGENSFRSLTWPPSGSPSHAGTTPPENGLSEHPCETEQINAKRKDTTSDKDDSLGSQQTNEQCAQKAEPTESCEQIAVQVNNGDAGREMPCPLPCDEESPEAELHNHGIQINSCSVRLVDIKKEKPFSNSKVECQAQARTHHNQASDIIVISSEDSEGSTDVDEPLEVFISAPRSEPVINNDNPLESNDEKEGQEATCSRPQIVPEPMDFRKLSTFRESFKKRVIGQDHDFSESSEEEAPAEASSGALRSKHGEKAPMTSRSTSTWRIPSRKRRFSSSDFSDLSNGEELQETCSSSLRRGSGSQPQEPENKKCSCVMCFPKGVPRSQEARTESSQASDMMDTMDVENNSTLEKHSGKRRKKRRHRSKVNGLQRGRKKDRPRKHLTLNNKVQKKRWQQRGRKANTRPLKRRRKRGPRIPKDENINFKQSELPVTCGEVKGTLYKERFKQGTSKKCIQSEDKKWFTPREFEIEGDRGASKNWKLSIRCGGYTLKVLMENKFLPEPPSTRKKRILESHNNTLVDPCEEHKKKNPDASVKFSEFLKKCSETWKTIFAKEKGKFEDMAKADKAHYEREMKTYIPPKGEKKKKFKDPNAPKRPPLAFFLFCSEYRPKIKGEHPGLSIDDVVKKLAGMWNNTAAADKQFYEKKAAKLKEKYKKDIAAYRAKGKPNSAKKRVVKAEKSKKKKEEEEDEEDEQEEENEEDDDK
STORAGE
Store at –80°C. Avoid repeated freezing/thawing cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
FORMULATION
Liquid in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, Protease inhibitor, pH8.0).
APPLICATION
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
IMMUNOLOGICAL FUNCTION
Together with PML, Sp100 is a major constituent of the PML bodies, a subnuclear
organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis.
Autoantibody against Sp100 are suggested as asignificant role in the diagnosis of primary biliary cirrhosis.