PL-7 (Human Threonyl-tRNA Synthetase)
Origin:
Recombinant
Cat. No.:
41480
Tag:
N-terminal 6xHis
Size:
0.1 mg
Source:
Spodoptera frugiperda Sf9
Purity:
>90%
Other Names:
TARS, PL 7, PL7
Species:
Human
INTRODUCTION
During mRNA translation at ribosomes, specific amino-acyl-tRNA synthetases are required for the covalently attachment between tRNAs and different amino acids.
Threonyl-tRNA synthetase (TARS, PL-7) is specific for the amino acid threonine.
PL-7 is recognized by PL-7 autoantibodies which is present in a subset of patients with polymyositis and dermatomyositis.
IMMUNOLOGICAL FUNCTION
As an autoantigen, PL-7 binds with IgG-type human auto-antibodies.
AMINO ACID SEQUENCE
MSYYHHHHHHDYDIPTTENLYFQGAFEEKASSPSGKMGGEEKPIGAGEEKQKEGGKKKNKEGSGDGGRAELNPWPEYIYTRLEMYNILKAEHDSILAEKAEKDSKPIKVTLPDGKQVDAESWKTTPYQIACGISQGLADNTVIAKVNNVVWDLDRPLEEDCTLELLKFEDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFSSLEALCKKIIKEKQAFERLEVKKETLLAMFKYNKFKCRILNEKVNTPTTTVYRCGPLIDLCRGPHVRHTGKIKALKIHKNSSTYWEGKADMETLQRIYGISFPDPKMLKEWEKFQEEAKNRDHRKIGRDQELYFFHELSPGSCFFLPKGAYIYNALIEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHYSENMFSFEVEKELFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEVWDQAEKQLENSLNEFGEKWELNSGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTYVSHDGDDKKRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCDEYAQKVRQQFHDAKFMADIDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKISGTVNIRTRDNKVHGERTISETIERLQQLKEFRSKQAEEEF
APPLICATIONS
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
FORMULATION
Liquid in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, Protease inhibitor, pH8.0).
DESCRIPTION
Expressed in insect Sf9 cells with total 747 AA. Mw: 86.4 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
STORAGE
Store at –80°C. Avoid repeated freezing/thawing cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.