LC1 (Human Formiminotransferase Cyclodeaminase)
Origin:
Recombinant
Cat. No.:
41A052
Tag:
N-terminal 6xHis
Size:
0.1 mg
Source:
Spodopterafrugiperda Sf9
Purity:
>90%
Other names:
LC1, FTCD
Species:
Human
INTRODUCTION
LC1 is an enzyme which catalyzes the conversion of formiminoglutamate and tetrahydrofolate into formiminotetrahydrofolate and glutamate. As a bio-functional enzyme of tetrahydrofolate synthesis, it is the target antigen of anti-LC1 (liver cytosol antigen type 1) autoantibodies. Presence of LC1 autoantibodies is a marker for type 2 autoimmune hepatitis.
DESCRIPTION
Expressed in insect Sf9 cells with a total 565 AA.
Mw: 61.9KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, extra 25 AA (highlighted).
Recombinant antigen for research use or manufacturing only.
AMINO ACID SEQUENCE
MSYYHHHHHHDYDIPTTENLYFQGASQLVECVPNFSEGKNQEVIDAISGAITQTPGCVLLDVDAGPSTNRTVYTFVGPPECVVEGALNAARVASRLIDMSRHQGEHPRMGALDVCPFIPVRGVSVDECVLCAQAFGQRLAEELDVPVYLYGEAARMDSRRTLPAIRAGEYEALPKKLQQADWAPDFGPSSFVPSWGATATGARKFLIAFNINLLGTKEQAHRIALNLREQGRGKDQPGRLKKVQGIGWYLDEKNLAQVSTNLLDFEVTALHTVYEETCREAQELSLPVVGSQLVGLVPLKALLDAAAFYCEKENLFILEEEQRIRLVVSRLGLDSLCPFSPKERIIEYLVPERGPERGLGSKSLRAFVGEVGARSAAPGGGSVAAAAAAMGAALGSMVGLMTYGRRQFQSLDTTMRRLIPPFREASAKLTTLVDADAEAFTAYLEAMRLPKNTPEEKDRRTAALQEGLRRAVSVPLTLAETVASLWPALQELARCGNLACRSDLQVAAKALEMGVFGAYFNVLINLRDITDEAFKDQIHHRVSSLLQEAKTQAALVLDCLETRQE
STORAGE
Store at –80°C. Avoid repeated freezing/thawing cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
FORMULATION
Liquid in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, Protease inhibitor, pH8.0).
APPLICATION
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
IMMUNOLOGICAL FUNCTION
As an autoantigen, LC1 binds with IgG-type human auto-antibodies.