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Micro-albumin (mALB) Turbidimetric Immunoassay Kit

Micro-albumin (mALB) Turbidimetric Immunoassay Kit

SKU: 51960
  • INTRODUCTION

    Micro-albumin(mALB), also known as urine albumin, is an important blood protein predominantly made by liver tissue that balances the blood osmotic pressure and transports hormones, vitamins, and substances like calcium throughout human body. Under normal circumstances, micro- albumin with 66kDa molecular weight is too large to cross the glomerular basal membrane. During kidney diseases, the pathological damage to the glomerular membrane leads to changes in permeability, and eventually causes micro-albumin excess excretion in urine. Therefore, micro-albumin is a sensitive and decisive biomarker for kidney injury. Micro-albumin test can timely detect kidney disease at early stage and help doctors make a right diagnosis to prevent deterioration. A higher level of micro-albumin in urine indicated the more severe kidney damage.

    The reference of micro- albumin in health human is less than 20mg/L, and Reversible renal injury is among 20mg/L to 200mg/L, and Irreversible renal injury (renal failure or uremia) is above 200mg/L.

  • PRINCIPLE OF THE ASSAY

    This assay is a turbidimetric immunoassay for the quantitative measurement of micro-albumin in human urine. A standard or sample is added into a cuvette and mixed with the reaction buffer R1. After a short incubation, the test reagent R2, which is a suspension of microparticles coated with an antibody highly specific to micro-albumin, is added into the cuvette and mixed. The presence of micro-albumin in the standard or sample causes the immune-particles to aggregate. The extent to which the microparticles aggregate is quantified by the amount of light scattering measured as absorbance by a chemistry analyzer. The concentration of Micro-albumin in unknown samples can be interpolated from a reference curve using the standards provided.

  • PERFORMANCE OF THE ASSAY

    TYPICAL STANDARD CURVE

    mALB (mg/L)

    Absorbance (570 nm)

    0

    -0.0023

    12.5

    0.0418

    25

    0.0909

    50

    0.1833

    100

    0.3037

    200

    0.4356

  • ASSAY PROCEDURE

    Assay procedures may vary depending on the automated chemistry analyzer to be used. A general example of assay procedures is stated as follow:

    1. Dispense 150µl of R1 into a clean cuvette

    2. Add 1.5µl of urine sample or Micro-albumin calibrator and incubate at 37˚C for 5 minutes

    3. Further add 50µl of R2

    4. Read change of absorbance at 570 nm for 8 minutes after the addition of R2

    5. Calculate the concentration of Micro-albumin in unknown sample by interpolation from a reference curve using the standards provided

  • PACKING SPECIFICATIONS

    Cat. No.

    Size

    Approximately tests

    51960-05

    R1: 15ml, R2: 5ml

    100

    51960-10

    R1: 30ml, R2: 10ml

    200

    51960-20

    R1: 60ml, R2: 20ml

    400

    51960-50

    R1: 150ml, R2: 50ml

    1000

    51960-100

    R1: 300ml, R2: 100ml

    2000

    For bulk order, please enquire

  • OTHER MATERIALS REQUIRED

    Product           

    Catalog           Number

    Format

    Method    

    Calibrator                 

    51960-S1

    5 x 1ml

     

    Control

    51960-C1

    2 x 1ml

     
PriceFrom $220.00

The currency used on this website is United States Dollars (USD). All prices are displayed and transactions are processed in USD. For orders outside Hong Kong or if you would like to have other currencies available, please contact us for a quote.

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