Adiponectin is an adipokine exclusively expressed in adipose tissues with potent anti-diabetic, anti-atherogenic and anti-inflammatory functions.
In humans, decreased serum adiponectin levels are associated with increased body mass index (BMI), decreased insulin sensitivity, less favourable plasma lipid profiles, increased inflammation and increased risk for the development of type 2 diabetes, hypertension and coronary heart diseases

This assay is a sandwich ELISA using affinity-purified polyclonal antibodies against mouse adiponectin. The immunoplate is pre-coated with anti-mouse adiponectin capture antibody. Standards and samples are pipetted into the wells and any mouse adiponectin present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked polyclonal antibody specific for mouse adiponectin is added to the wells. After a final wash step, an HRP substrate solution is added and colour develops in proportion to the amount of mouse adiponectin bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse adiponectin, the unknown sample concentration can be interpolated from a reference curve included in each assay.

A. Typical representation of standard curve

The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.

Adiponectin (ng/mL)	Absorbance (450 nm)	Blanked Absorbance
0	0.064	0
3.12	0.105	0.041
6.25	0.151	0.087
12.5	0.257	0.193
25	0.467	0.403
50	0.801	0.737
100	1.463	1.399
200	2.393	2.329

 

B. Sensitivity

The lowest level of mouse adiponectin that can be detected by this assay is 3.12 ng/mL.

 

C. Specificity

The antibodies used in this assay are specific to mouse adiponectin and do not cross-react with human adiponectin, and other cytokine or hormone molecules.

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