Autotaxin (ATX, ENPP2) is a secreted glycoprotein with phosphodiesterase (PDE) activity. It is one of the member in the nucleotide pyrophosphatase/phosphodiesterase family (NPPs) family. ATX has lysophospholipase D activity that converts lysophosphatidylcholine into LPA, and it was originally identified as a tumor cell-motility-stimulating factor. LPA, which specifically binds to G protein-coupled receptors (GPCR), plays a wide range of biological activities, including cell hyperproliferation, which may contribute to oncogenesis and metastasis. ATX has been to be overexpressed in many tumor cells, and thus plays an important role in tumor development and metastasis. In addition, ATX also plays an important role in nervous and immune systems.

Expressed in HEK293 cells with total 871 AA. Mw: 100.1 KDa (calculated).

C-terminal 6xHis-tag, 9 extra AA (highlighted).

Recombinant protein for research use or manufacturing only.

MARQGCFGSYQVISLFTFAIGVNLCLGFTASRIKRAEWDEGPPTVLSDSPWTNTSGSCKGRCFELQEVGPPDCRCDNLCKSYSSCCHDFDELCLKTARGWECTKDRCGEVRNEENACHCSEDCLSRGDCCTNYQVVCKGESHWVDDDCEEIRVPECPAGFVRPPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHAPYMRPVYPTKTFPNLYTLATGLYPESHGIVGNSMYDPVFDATFHLRGREKFNHRWWGGQPLWITATKQGVRAGTFFWSVSIPHERRILTILQWLSLPDNERPSVYAFYSEQPDFSGHKYGPFGPEMTNPLREIDKTVGQLMDGLKQLKLHRCVNVIFVGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRPKIPNNLKYDPKAIIANLTCKKPDQHFKPYMKQHLPKRLHYANNRRIEDLHLLVERRWHVARKPLDVYKKPSGKCFFQGDHGFDNKVNSMQTVFVGYGPTFKYRTKVPPFENIELYNVMCDLLGLKPAPNNGTHGSLNHLLRTNTFRPTLPEEVSRPNYPGIMYLQSDFDLGCTCDDKVEPKNKLEELNKRLHTKGSTEERHLLYGRPAVLYRTSYDILYHTDFESGYSEIFLMPLWTSYTISKQAEVSSIPEHLTNCVRPDVRVSPGFSQNCLAYKNDKQMSYGFLFPPYLSSSPEAKYDAFLVTNMVPMYPAFKRVWTYFQRVLVKKYASERNGVNVISGPIFDYNYNGLRDIEDEIKQYVEGSSIPVPTHYYSIITSCLDFTQPADKCDGPLSVSSFILPHRPDNDESCNSSEDESKWVEELMKMHTARVRDIEHLTGLDFYRKTSRSYSEILTLKTYLHTYESEISYYHHHHHH

Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.  Avoid repeated freezing/thawing cycles.

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Lyophilized at 1 mg/mL in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).

ELISA and Western Blotting.

<0.01 EU per 1 μg of the protein by the LAL method.

Measured by enzymatic ability to cleave Bis (p- Nitrophenyl) Phosphate (BPNPP).

The specific activity of mouse ATX is > 5,000 pmol/min/μg

* Specific Activity (pmol/min/µg) = Adjusted Vmax* (OD/min) x Conversion Factor (pmol/OD)/Amount of Enzyme (µg)

1. Qiu H, Song E, Hu Y, Li T, Ku KC, Wang C, Cheung BMY, Cheong LY, Wang Q, Wu X, Hoo RLC, Wang Y, Xu A. Hepatocyte-Secreted Autotaxin Exacerbates Nonalcoholic Fatty Liver Disease Through Autocrine Inhibition of the PPARα/FGF21 Axis. Cell Mol Gastroenterol Hepatol. 2022;14(5):1003-1023.