SSA/Ro52 (Human Tripartite Motif Containing 21)
Origin:
Recombinant
Cat. No.:
41590
Tag:
N-terminal 6xHis
Size:
0.1 mg
Source:
Spodoptera frugiperda Sf9
Purity:
>85%
Other Names:
TRIM21, SSA/Ro52, Ro52
Species:
Human
INTRODUCTION
Ro/SSA ribonucleoproteins are involved in RNA quality control and cellular stress response to UV irradiation, and comprise of a single polypeptide and four small RNA molecules including the 52 kDa Ro protein. Ro/SSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus.
IMMUNOLOGICAL FUNCTION
As an autoantigen, SSA/Ro52 binds with IgG-type human auto-antibodies
AMINO ACID SEQUENCE
MSYYHHHHHHDYDIPTTENLYFQGAASAARLTMMWEEVTCPICLDPFVEPVSIECGHSFCQECISQVGKGGGSVCPVCRQRFLLKNLRPNRQLANMVNNLKEISQEAREGTQGERCAVHGERLHLFCEKDGKALCWVCAQSRKHRDHAMVPLEEAAQEYQEKLQVALGELRRKQELAEKLEVEIAIKRADWKKTVETQKSRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKKMLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLGAQHFHSGKHYWEVDVTGKEAWDLGVCRDSVRRKGHFLLSSKSGFWTIWLWNKQKYEAGTYPQTPLHLQVPPCQVGIFLDYEAGMVSFYNITDHGSLIYSFSECAFTGPLRPFFSPGFNDGGKNTAPLTLCPLNIGSQGSTDY
APPLICATIONS
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
FORMULATION
Liquid in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, Protease inhibitor, pH8.0).
DESCRIPTION
Expressed in insect Sf9 cells with a total of 499 AA.
Mw: 57.1 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
STORAGE
Store at –80°C. Avoid repeated freezing/thawing cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.